WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with... WebПодтвержденная временем надежность профессионалов. Мы работаем в области аудита и консалтинга с 1997 года, когда было создано ЗАО «Аудиторская фирма «ФОРПОСТ», в 2005 году оно было преобразовано в ООО «Аудиторская ...
Integrative analysis of aberrant Wnt signaling in hepatitis B virus ...
Web22. sep 2024 · The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously. It has broad applications across various fields of cell and molecular biology—wherever you want to … WebThe LEADING LIGHT ® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format. The system contains an engineered 3T3 mouse fibroblast cell line, which expresses the firefly luciferase reporter gene under the control of Wnt-responsive promoters (TCF/LEF). chlorhexidine mouthwash tongue stain
Top Flash Promega Bioz
WebPočet riadkov: 10 · TOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal … Web15. aug 2013 · Using lentiviral wild-type (Top) and mutant (Fop) TCF/LEF reporters, we investigated the status of Wnt signaling in cells derived from several types of human cancer. As a result of this screen, we identified up-regulated TCF/LEF activity in different hematopoietic tumor cells, including Ramos, K562 and Jurkat ( Figure 1A ). WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame. - Find MSDS or SDS, a COA, data sheets and more information. chlorhexidine mouthwash teeth staining