Web16 Jun 2014 · The cloning vector pMD18-T was purchased from TakaRa, and the expression vector pBV220 was obtained from the Changchun Institute of Biological Products. All restriction enzymes and the Expand High Fidelity PCR system were purchased from TakaRa. The sequences of the PCR products used for cloning were confirmed by sequencing at … Webenzyme digestion reaction was terminated by incubation at 65°C for 10 min. Then, the ligation reaction was proceeded in a final volume of 40 μl mixture, which contains 1 unit T4 DNA ligase, 0.2 mM ATP, 5 pm EcoRI adapters and 50 pm HpaII/MspI adapters for additional 6 h at 20°C (Table 1).

Cloning Genes-of-Interest into a Plasmid Vector - Sigma-Aldrich

WebLow birth-weight (LBW) neonates exhibit a lower growth rate and impaired intestinal development. However, the reasons for abnormal development of small intestine in LBW piglets have not been widely studied. The present study focused on the redox status and mitochondrial morphology and functions of the small intestine in LBW newborn piglets. … Web11 Apr 2024 · PrimeSTAR (TaKaRa, China) was used to amplify the entire coding sequence of SzCYP6MS family genes (CYP6MS1, CYP6MS5, CYP6MS6, CYP6MS8, and CYP6MS9) using polymerase chain reaction (PCR). ... (China) contains two convergent T7 polymerase promoters in opposite orientation, was digested using NotI and KpnI restriction enzymes. … how to add text into your video

T4 Dna Ligase Enzyme TaKaRa Bioz

Web26 Apr 2024 · TaKaRa ExTaq™ DNA polymerase (Takara Bio Inc., Kusatsu, Japan) was used in polymerase chain reactions (PCR) required for the manipulation of DNA while GoTaq ® DNA Polymerase (Promega Corporation, Madison, WI, USA) was used for screening and confirmation PCR reactions. The primers and the PCR programs used in this study are … WebBed & Board 2-bedroom 1-bath Updated Bungalow. 1 hour to Tulsa, OK 50 minutes to Pioneer Woman You will be close to everything when you stay at this centrally-located … met office great yarmouth forecast