Phosphomotif antibodies
WebFeb 2, 2024 · Antibody buffer and 0.5 μg of antibody were added to samples and incubated overnight at 4°C. After washing beads, 2.5 μl of pAG-NMase was added and incubated for 10 min at RT. After washing, 1 μl of chromatin digest additive was added and incubated for 2 hours at 4°C. Stop buffer and 0.5 ng of Spike-in DNA were then added and incubated for ... WebApr 11, 2024 · Ph2AX antibody was initially tested on cisplatin-treated and untreated iKras PDAC cells embedded in agarose, and an optimal dilution of 1:400 was determined for the detection of DNA damage cells by IHC staining. Primary and secondary OmniMap HRP-conjugated antibodies were used in 100 μl volumes, for 60 and 16 min, respectively.
Phosphomotif antibodies
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WebUnlike phosphoantibodies raised against a single amino acid sequence, phosphomotif antibodies (first developed by Cell Signaling Technology) recognize many different phosphoproteins, ... WebNov 11, 2013 · Here we have used phosphomotif antibodies and mass spectrometry (MS) to investigate the effects of MEK/PI3K dual inhibition during the period immediately preceding cell death. Upon treatment, melanoma cell lines responded by dramatically increasing phosphorylation on proteins containing a canonical DNA damage-response (DDR) motif, …
WebMay 13, 2015 · The ability of anti-mHttexon1 antibodies and antibody fragments to reduce the aggregation and associated neurotoxicity has proven to be strongly dependent on the targeted epitope . Specifically targeting the Htt sequence between 1 and 17, which has been shown to play a key role in aggregation and HD pathology, seems to be an obvious choice. WebApr 13, 2024 · Figure 1.Expression levels of obscurin in wild-type and Obscn-ΔIg58/59 atria.(A,A’) Immunoblotting (A) and relative quantification (A’) of giant obscurin in wild-type 12-month-old left ventricles (LV) and atrial lysates utilizing antibodies to Ig67 did not reveal significant expression differences; t-test, p = 0.18.(B) Immunoblotting of giant obscurin in …
WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and … WebFeb 1, 2016 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and …
WebApr 1, 2009 · Phosphomotif antibody 5557 is phosphospecific and detects MAPK phosphorylation events.A, sequence of phosphopeptide library containing two …
Webever, phosphomotif antibodies have not been used so far for large scale proteomics identification of kinase substrates. To discover novel MAPK targets at the synapse, we raised a phosphospecific antibody against a peptide library repre-senting the MAPK consensus phosphorylation motif. Using this antibody we affinity-purified putative MAPK ... optus fax test numberWebDec 8, 2015 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and suggest signaling pathways by which... optus fetch box priceWebAntibodies—A key reagent for this study is the antibody for the Akt phosphomotif RXRXXpS/T, where X is any amino acid. This antibody, which is referred as the PAS antibody (phospho Akt substrate), was purchased from Cell Signaling Technology, Beverly, MA (catalog num-ber 9611). It is an affinity-purified rabbit antibody that has been shown optus fetch and internetWebThis study describes a method for the identification of the substrates of specific serine kinases. An antibody specific for the phosphomotif generated by the kinase is used to … portsmouth archery score sheetWebFeb 11, 2013 · The scaffolding adapter protein Gab2 (Grb2-associated binder) participates in the signaling response evoked by various growth factors and cytokines. Gab2 is … portsmouth apprenticeshipsWebPhosphomotif Antibody Generation and Purification Phosphorylated and non-phosphorylated peptide libraries were chemically synthesized with C-terminal cysteine or … optus fetch boxWebJan 9, 2014 · After 2D-DIGE analysis, changed protein spots were characterized for reactivity to ERK phosphomotif antibodies and identified by MS. Various types of phosphomotif … optus fetch box stuck on starting