Peasy-blunt载体序列
WebDec 23, 2024 · 质粒的接合转移 :是细菌遗传物质转移的一个重要方式。. 在质粒转移过程中,供体菌和受体菌通过结合作用紧密接触,质粒从供体细胞向受体转移,同时进行质粒复制。. 按能否自主转移,可以将天然存在的质粒分为转移型质粒和非转移型质粒两大类。. 这里要 ... WebpEasyBlunt用的这种T载体分好几种,有的是不带“blunt”的,有的是带blunt的,同时还有带多克隆位点还没有多克隆位点的。 用于克隆,但是它的连接片段须得是用保真酶(比 …
Peasy-blunt载体序列
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Web全式金生物pEASY-Blunt Cloning Kit技术使用说明书. 全式金生物pEASY-Blunt Cloning Kit技术使用说明书_生物学_自然科学_专业资料。全式金生物的pEASY-Blunt Cloning Kit产品的技术使用说明书,仅作为学术研究,不能作为... 实验总结-3’race. pEASY-Blunt Simple Cloning Kit购 … WebpEASYT1载体中文说明书. -3′端悬垂胸腺嘧啶 (T)可用于TA克隆. -拓扑异构酶与载体相互偶联(活化载体). TaqDNA聚合酶具有类似脱氧核糖核酸末端转移酶 (TdT)的功能,可在新合成双链产物的3’端加上一个碱基。. 尽管4种碱基均可被聚合到3’端,但Taq酶对dATP的聚合 ...
WebManual Vector Information MSDS COA. Inquire Now Buy Now Add to Cart Free Sample Avaliable. Product Details. pEASY® -Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white … WebFigure Legend Snippet: Kinetic properties of recombinant PiGSTd1. (A) Purification of recombinant pEasy-Blunt-E1-PiGSTd1. Induced: the crude extracts from the bacterial pellets with 1mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. 1–4: samples eluted with binding buffers containing 50, 100, 150, and 200mM imidazole, respectively.
WebpEASY-Blunt Cloning Kit使用说明书. 本产品仅供研究,不用于临床诊断。. Resistant Chemically Competent Cell。. Expected PCR. product size: 700 bp。. 产品说明 pEASY®- … Web一、载体概念. 1. 载体,DNA的搬运工. 载体:在基因工程重组DNA技术中,将DNA片段(目的基因)转移至受体细胞的一种能自我复制的DNA分子。. 2. 载体,基因密码承载者. 载体,小人物的大作用. ① 分子较小;. ② 尽可能多限制酶切割位点且单一;.
WebOct 22, 2016 · pEASY-Blunt clonning vector 载体说明书WORD版.doc 22页. pEASY-Blunt clonning vector 载体说明书WORD版.doc. 22页. 内容提供方 : me54545. 大小 : 419 KB. …
box and pierceWeb产品详情介绍. pEASY®-Blunt Cloning Vector包含LacZ基因,在含有IPTG和X-gal的平板培养基上,可进行蓝白斑筛选,适用于平端克隆。. gun shows victorville caWebManual Vector Information MSDS COA. Inquire Now Buy Now Add to Cart Free Sample Avaliable. Product Details. pEASY® -Blunt Cloning Kit is designed for cloning and … box and pierce’s q 检验WebAug 7, 2013 · 产品说明pEASY-T1CloningKits适用于TA克隆.-快速克隆基因,仅需要5分钟反应时间。. 对照插入片段克隆效率可达95%以上。. 包含LacZ基因,在含有IPTG,X-gal的平板培养基上,可进行蓝白斑筛选。. 工作原理pEASY-T1CloningKits以线性的形式提供:-3′端悬垂胸腺嘧啶 (T)可用于TA ... box and piiWebpEASY-Blunt E1 (linearized) pHY300PLK. pSMART HCAmp. T7 promoter. pEASY-Blunt E2 (linearized) pICOz. pSMART HCAmp (linearized) tac promoter. pEASY-Blunt Simple (linearized) pInvRecA. pSMART HCKan. trc promoter. pEASY-Blunt Zero (linearized) pJAZZ-OC. pSMART HCKan (linearized) phiX174. pEASY-Blunt3 (linearized) box and pin threadWebpEASY-Blunt3 (linearized) Sequence and Map. Cloning vector for blunt-ended PCR products. The cloned PCR product can be excised with EcoRI or NotI. Cloning vector for blunt-ended … gun shows virginiaWebApr 13, 2024 · As a starchy and edible tropical plant, cassava (Manihot esculenta Crantz) has been widely used as an industrial raw material and a dietary source. However, the metabolomic and genetic differences in specific germplasms of cassava storage root were unclear. In this study, two specific germplasms, M. esculenta Crantz cv. sugar cassava … gun shows virginia 2021