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Peasy-blunt载体序列

Webpeasy 系列克隆载体; 相关产品; 克隆感受态细胞; 点突变; 核酸纯化 . 基因组dna纯化; 质粒dna纯化; dna产物纯化; rna纯化; 相关产品; 二代测序 . dna建库; rna建库; 相关产品; mngs; … WebDownload pEASY-Blunt E1 (linearized).dna file. Download Plasmid Open in SnapGene. SnapGene. SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. Fast accurate construct design for all major molecular cloning techniques;

PmACRE 基因的克隆及遗传转化拟南芥

WebDec 23, 2024 · 质粒的接合转移 :是细菌遗传物质转移的一个重要方式。. 在质粒转移过程中,供体菌和受体菌通过结合作用紧密接触,质粒从供体细胞向受体转移,同时进行质粒复 … WebAug 3, 2016 · pEASY- T1 Cloning Kits在3’ 端提供一个悬垂的T 碱基,这样的结构使PCR片段可以高效的和载体进行连接。. 牛痘拓扑异构酶I型可以与DNA双链 的特定位置结合并切割特异位点5’- (C/T)CCTT-3’, 与3‘T的磷酸基团性成共价键,并切断其中 一条DNA链,使DNA解链。. … box and paperie https://almadinacorp.com

pEASY-Blunt Cloning Kit使用说明书_文档下载

WebpEASY-Blunt clonning vector 载体说明书WORD版. LacZfragment: bases 1-548. M13 reverse priming site: bases 205-221. Multiple cloning site: bases 234-358. T7 promoter/priming … WebQuantity:. pEASY® - Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion. •5 minutes fast ligation of Pfu-amplified PCR products. •Ampicillin resistance gene for selection. Web产品详情介绍. pEASY®-Blunt Simple Cloning Vector消除了pEASY®-Blunt Cloning Vector上的多克隆位点,克隆后的PCR产物无法使用pEASY®-Blunt Cloning Vector上多克隆位点的限制性内切酶切下。. 包含LacZ基因,在含有IPTG和X-gal的平板培养基上,可进行蓝白斑筛选, 适用于平端克隆。. gun shows ventura county

谁做过pEASY-Blunt Cloning啊?谁做过平末端连接啊? - 分子生物

Category:一种表达鸡传染性法氏囊病病毒VP2基因的重组火鸡疱疹病毒株制 …

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Peasy-blunt载体序列

pEASY-Blunt clonning vector 载体说明书WORD版.doc - 原创力文档

WebDec 23, 2024 · 质粒的接合转移 :是细菌遗传物质转移的一个重要方式。. 在质粒转移过程中,供体菌和受体菌通过结合作用紧密接触,质粒从供体细胞向受体转移,同时进行质粒复制。. 按能否自主转移,可以将天然存在的质粒分为转移型质粒和非转移型质粒两大类。. 这里要 ... WebpEasyBlunt用的这种T载体分好几种,有的是不带“blunt”的,有的是带blunt的,同时还有带多克隆位点还没有多克隆位点的。 用于克隆,但是它的连接片段须得是用保真酶(比 …

Peasy-blunt载体序列

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Web全式金生物pEASY-Blunt Cloning Kit技术使用说明书. 全式金生物pEASY-Blunt Cloning Kit技术使用说明书_生物学_自然科学_专业资料。全式金生物的pEASY-Blunt Cloning Kit产品的技术使用说明书,仅作为学术研究,不能作为... 实验总结-3’race. pEASY-Blunt Simple Cloning Kit购 … WebpEASYT1载体中文说明书. -3′端悬垂胸腺嘧啶 (T)可用于TA克隆. -拓扑异构酶与载体相互偶联(活化载体). TaqDNA聚合酶具有类似脱氧核糖核酸末端转移酶 (TdT)的功能,可在新合成双链产物的3’端加上一个碱基。. 尽管4种碱基均可被聚合到3’端,但Taq酶对dATP的聚合 ...

WebManual Vector Information MSDS COA. Inquire Now Buy Now Add to Cart Free Sample Avaliable. Product Details. pEASY® -Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white … WebFigure Legend Snippet: Kinetic properties of recombinant PiGSTd1. (A) Purification of recombinant pEasy-Blunt-E1-PiGSTd1. Induced: the crude extracts from the bacterial pellets with 1mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. 1–4: samples eluted with binding buffers containing 50, 100, 150, and 200mM imidazole, respectively.

WebpEASY-Blunt Cloning Kit使用说明书. 本产品仅供研究,不用于临床诊断。. Resistant Chemically Competent Cell。. Expected PCR. product size: 700 bp。. 产品说明 pEASY®- … Web一、载体概念. 1. 载体,DNA的搬运工. 载体:在基因工程重组DNA技术中,将DNA片段(目的基因)转移至受体细胞的一种能自我复制的DNA分子。. 2. 载体,基因密码承载者. 载体,小人物的大作用. ① 分子较小;. ② 尽可能多限制酶切割位点且单一;.

WebOct 22, 2016 · pEASY-Blunt clonning vector 载体说明书WORD版.doc 22页. pEASY-Blunt clonning vector 载体说明书WORD版.doc. 22页. 内容提供方 : me54545. 大小 : 419 KB. …

box and pierceWeb产品详情介绍. pEASY®-Blunt Cloning Vector包含LacZ基因,在含有IPTG和X-gal的平板培养基上,可进行蓝白斑筛选,适用于平端克隆。. gun shows victorville caWebManual Vector Information MSDS COA. Inquire Now Buy Now Add to Cart Free Sample Avaliable. Product Details. pEASY® -Blunt Cloning Kit is designed for cloning and … box and pierce’s q 检验WebAug 7, 2013 · 产品说明pEASY-T1CloningKits适用于TA克隆.-快速克隆基因,仅需要5分钟反应时间。. 对照插入片段克隆效率可达95%以上。. 包含LacZ基因,在含有IPTG,X-gal的平板培养基上,可进行蓝白斑筛选。. 工作原理pEASY-T1CloningKits以线性的形式提供:-3′端悬垂胸腺嘧啶 (T)可用于TA ... box and piiWebpEASY-Blunt E1 (linearized) pHY300PLK. pSMART HCAmp. T7 promoter. pEASY-Blunt E2 (linearized) pICOz. pSMART HCAmp (linearized) tac promoter. pEASY-Blunt Simple (linearized) pInvRecA. pSMART HCKan. trc promoter. pEASY-Blunt Zero (linearized) pJAZZ-OC. pSMART HCKan (linearized) phiX174. pEASY-Blunt3 (linearized) box and pin threadWebpEASY-Blunt3 (linearized) Sequence and Map. Cloning vector for blunt-ended PCR products. The cloned PCR product can be excised with EcoRI or NotI. Cloning vector for blunt-ended … gun shows virginiaWebApr 13, 2024 · As a starchy and edible tropical plant, cassava (Manihot esculenta Crantz) has been widely used as an industrial raw material and a dietary source. However, the metabolomic and genetic differences in specific germplasms of cassava storage root were unclear. In this study, two specific germplasms, M. esculenta Crantz cv. sugar cassava … gun shows virginia 2021